Thailand Institute of Scientific and Technological Research (TISTR), CP ALL Plc. has studied effects of holy basil extracted on fat reduction, hepatic cell protection, and cancer cell suppression

Thailand Institute of Scientific and Technological Research (TISTR), Ministry of Higher Education, Science, Research and Innovation (MHESI) has signed collaboration agreement with CP ALL Plc. on research and development of holy basil extracted using screened out holy basil parts (peduncle, stalk, and stem) from ready-to-eat food factories and its effects on fat reduction, hepatic cell protection, and cancer cell suppression. This research project is persist in our ambition “giving and sharing opportunities from all”, and objectives for continuous development of society, community, and environment.

Dr. Chutima  Eamchotchawalit, Governor, Thailand Institute of Scientific and Technological Research (TISTR), Ministry of Higher Education, Science, Research and Innovation (MHESI) has referred to the institute’s policy and objective of the collaboration project that TISTR has strived to conduct research work: develop; analyze; test; verify, focusing on technology transfer both commercial and social purposes as well as academic collaboration. These factors are crucial for development and driving innovative-social economic and competitiveness. In order to integrally supportive co-operation with governmental agencies and private sector and systematically develop “Science Technology Innovation” (STI) networking. The STI is an important foundation for effectively solving socio-economic issues. This is an alignment with CP ALL Plc. mission, “always response costumers’ need with quality and innovative products and services”

“With the objectives of the study are aiming for utilizing remaining holy basil, the herbal specialize innovative center[KS1] , TISTR will perform study together with CP ALL Pcl. by bring more than 56 years response customer demands experience and research capacity on analytic herbal products services that has international accepted standards, aiming for successful of the research project” the Governor said

“Holy basil” is an herbal that has unique aromas. It is one of Thais’ all-time favorite ingredients using for top dishes such as stir-fried basil.

Stir-fried basil with rice is a customers’ top hit menu at seven eleven store that is produced by “CP RAM”, manufacturer and seller ready-to-eat meals of CP ALL group. In production processes, basil leafs are separated using for raw material and other remaining parts are screened out. This inspired the idea to utilize all basil parts, creating social and economic values.

Mr.Wisade Wisidwinyoo, executive committee, CP ALL Plc., the company where established seven eleven store in Thailand, said Nowadays, holy basil is a main ingredient for ready-to-eat box meal and is considered as an economic plant that act an important role on food security. In addition, CP RAM, one of CP ALL group member, has studied searching a good smell and spicy taste cultivars which found the best local species. Under “famer partnership” project, CP RAM has supported and encouraged farmers surrounding the factories to plant the local basil, creating incomes for their family. Resulting in excellent basil leafs serve to consumer and leading to their health. However, there are remaining basil parts that need to be utilized.

“Advancement of utilizing reaming basil will create additional economic and social values, increase and stabilize farmer’s income as well as enhancing quality of life for entire society. Hence, collaboration with TISTR performing a research will be benefit our food products by using extracted volatile organic compound and benefit on medical and pharmaceutical sectors as well.” Mr.Wisade explains

This research collaboration is aligned with CP ALL Plc. mission, “always response costumers’ need with quality and innovative products and services” and creating innovation that drive the organization to sustainability, along with continuous development of society, community, and environment.

Progress of collaboration research project between The Thailand Institute of Scientific and Technological Research (TISTR) and CP ALL Plc. on effects of sweet basil extracted on fat reduction, hepatic cell protection, and cancer cell suppression

In 2020, the project has been tested the effects of sweet basil extracted on 4 types of cancer cells which are breast cancer cell line (MCF-7), epithelial cancer cell line (G-361), colorectal adenocarcinoma cell line (Caco-2), and cervical cancer cell line (HeLa). The project has also studied the effect of in vivo fat reduction, and cytotoxicity. The progress of the research has been reported to the CPALL’s executives committee and STIDO.

Tests of pharmacology effects of the extracted 6 samples (listed below)

  1. Basil branches extracted by 50% ethanol
  2. Basil blossom extracted by 50% ethanol
  3. Basil branches extracted by 75% ethanol
  4. Basil branches extracted by 95% ethanol
  5. Basil oil extracted by steam distillation method
  6. Basil oil extracted by Solvent-free microwave extraction (SFME) method

Pharmacology results

  1. Cancer cell suppression

Basil extracted 31.25-1,000 μg/ml has suppressed all tested types cancer cell at 20-80% rate. The basil oil has better effect than the extraction of basil braches (has similar results with Mitomycin C).

  • In vivo fat reduction
    • Basil branches extracted by 95% ethanol has the best effect among other samples on inhibition fat granules at 13%. The extraction concentrated at 200 μg/ml has effect on tri-glyceride reduction at 16%.
    • Steam distillation braches oil extracted has the best result among other samples on inhibition fat granules at 50%. The oil at 1% concentration has effect on tri-glyceride reduction at 47% which has similar effect with 100 μg/ml Simvastatin, control positive chemical, that inhibits fat granules and tri-glyceride at 42% and 47% respectively
  • Cytotoxicity

Results of cytotoxicity test on L 929 found that toxic levels of the extractions (from minimum to maximum) are Basil oil extracted by Solvent-free microwave extraction (SFME) method > Basil oil extracted by steam distillation method > Basil blossom extracted by 50% ethanol > Basil branches extracted by 50% ethanol > Basil branches extracted by 75% ethanol > Basil branches extracted by 95% ethanol, respectively